Bacteroides fragilis isolates compared by AP-PCR
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- 作者：Moraes ; Saulo Roni ; Gonç ; alves ; Reginaldo Bruno ; Mouton ; Christian ; Seldin ; Lucy ; Ferreira ; Maria Candida de Souza ; et. al.
- 关键词：Bacteroides fragilis ; AP-PCR ; genetic diversity ; Bacteroides fragilis ; AP-PCR ; diversité ; gé ; né ; tique ; AP-PCR ; arbitrarily primed PCR ; BHI ; brain-heart infusion (broth) ; PCR ; polymerase chain reaction ; RAPD ; random amplified polymorphic
- 刊名：Research in Microbiology
- 出版年：1999
- 出版时间：May, 1999
- 年：1999
- 卷：150
- 期：4
- 页码：257-263
- 全文大小：206 K
文摘
Bacteroides fragilis is a component of the normal intestinal flora and an important pathogen in nonintestinal endogenous infections. It has been associated with enteric infections and has already been detected in polluted water. In order to evaluate the genetic diversity of B. fragilis, a total of 31 isolates and two reference strains were examined. This collection included strains from nonintestinal infections , intestinal infections , intestinal microflora , aquatic environments , and the reference strains ATCC 25285 and ATCC 23745. DNA fingerprints were detected using two separate PCR reactions with different arbitrary primers. The computer-assisted system Taxotron® (Institut Pasteur, Dr P. Grimont) was used to analyze the profiles obtained and dendrograms were generated. By using a distance of 0.65 as the threshold, two clusters (hereafter referred to as genotypes I and II) were defined. Strains of differents origins could be distributed into both genotypes. We were unable to detect any obvious correlation between a given genotype and the specific disease or the source of the corresponding strains.