Enantioseparation of anionic analytes by non-aqueous capillary electrophoresis using quinine and quinidine derivatives as chiral counter-ions

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• 作者：Piette ; V. ; Lindner ; W. ; Crommen ; J.
• 关键词：Non-aqueous capillary electrophoresis ; Enantiomeric separation ; Derivatization electrophoresis ; Chiral selectors ; Amino acids
• 刊名：Journal of Chromatography A
• 出版年：2000
• 出版时间：October 13, 2000
• 年：2000
• 卷：894
• 期：1-2
• 页码：63-71
• 全文大小：741 K

文摘

A non-aqueous capillary electrophoretic method developed for the enantioseparation of N-protected amino acids has been applied to the investigation of five new quinine and quinidine derivatives as chiral selectors: 1-adamantyl carbamoylated quinine, 3,4-dichlorophenyl carbamoylated quinidine, allyl carbamoylated dihydroquinine, allyl carbamoylated dihydroquinidine and 1-methyl quininium iodide. The composition of the background electrolyte was 12.5 mM ammonia, 100 mM octanoic acid in an ethanol–methanol (60:40 v/v) mixture containing a 10 mM concentration of the chiral selector. Under these conditions, the enantioseparation of a series of various benzoyl, 3,5-dinitrobenzoyl and 3,5-dinitrobenzyloxycarbonyl amino acid derivatives was studied with respect to selectand–selector relationship and enantioselectivity.