Biosynthesis of selenium-containing polysaccharides with antioxidant activity in liquid culture of Hericium erinaceum

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• 作者：Eliza Malinowska ; Wojciech Krzyczkowski ; Franciszek Herold ; Grzegorz Ł ; apienis ; Joanna Ś ; lusarczyk ; Piotr Suchocki ; Mieczysł ; aw Kuraś ; Jadwiga Turł ; o
• 关键词：Polysaccharides ; Hericium erinaceum ; Selenium ; Antioxidant activity ; Mushroom ; Selenitetriglycerides ; Selol
• 刊名：Enzyme and Microbial Technology
• 出版年：2009
• 出版时间：6 May 2009
• 年：2009
• 卷：44
• 期：5
• 页码：334-343
• 全文大小：840 K

文摘

The effects of inorganic selenium (sodium selenite) and Selol containing selenitetriglycerides synthesized from sunflower oil on mycelial growth and selenium-containing extracellular (EPS) and intracellular (IPS) polysaccharides production were examined in shake flask cultures of Hericium erinaceum.

Unlike sodium selenite which inhibited mycelial growth, Selol increased in biomass production in a dose-dependent manner. Selol also dramatically enhanced EPS formation to 2.25 g/L which is 2.5–fold higher than in the control. Selenium content in EPS and IPS obtained from Selol-enriched medium reached a maximum of 4.89 and 4.69 mg/g, respectively.

The in vitro antioxidant activities of polysaccharides were evaluated by reducing power, inhibition of lipid peroxidation, and 1,1-diphenyl-dipicrylhydrazyl radicals scavenging assays. The selenium-containing EPS showed an excellent antioxidant activity correlated well with increasing concentrations.

The results suggested that selenium-containing EPS from H. erinaceum submerged culture should be explored as a novel selenium source in dietary supplements, with potent antioxidant properties.