Two-stage label-free aptasensing platform for rapid detection of Cronobacter sakazakii in powdered infant formula
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- 作者：Hong-Seok KimaAuthor Vitae ; Young-Ji KimaAuthor Vitae ; Jung-Whan Chona ; 1Author Vitae ; Dong-Hyeon KimaAuthor Vitae ; Jin-Hyeok YimaAuthor Vitae ; Hyunsook KimbAuthor Vitae ; Kun-Ho Seoa ; bracstu3@konkuk.ac.kr" class="auth_mail" title="E-mail the corresponding authorAuthor Vitae
- 关键词：AuNP ; gold nanoparticle ; BB ; binding buffer ; PIF ; powdered infant formula ; SELEX ; systematic evolution of ligands by exponential enrichment ; U.S. FDA ; United States Food and Drug Administration
- 刊名：Sensors & Actuators: B. Chemical
- 出版年：2017
- 出版时间：February 2017
- 年：2017
- 卷：239
- 期：Complete
- 页码：94-99
- 全文大小：1149 K
文摘
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A two-stage label-free aptasensing platform was established to detect C. sakazakii.
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This is the first report of an aptamer against C. sakazakii.
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This assay can detect C. sakazakii in PIF at a 7.1 × 103 CFU/mL detection limit.
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This assay requires only 30 min or less to complete after sample enrichment.
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This platform may be applicable for detecting other bacteria in real-world samples.