The efficient cell-SELEX strategy, Icell-SELEX, using isogenic cell lines for selection and counter-selection to generate RNA aptamers to cell surface proteins

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• 作者：Masaki Takahashi^a ; Eri Sakota^a ; Yoshikazu Nakamura^a ; ^b ; ^{nak@ims.u-tokyo.ac.jp}
• 关键词：RNA aptamer ; Isogenic cell-SELEX ; RNA silencing ; Cell surface proteins ; Integrin alpha V
• 刊名：Biochimie
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：131
• 期：Complete
• 页码：77-84
• 全文大小：1407 K
• 卷排序：131

文摘

An efficient cell-SELEX strategy is developed and named Icell-SELEX. Selection and counter-selection cells are manipulated by gene engineering. RNA aptamers to integrin alpha V on the cell surface were isolated.