Investigating piconewton forces in cells by FRET-based molecular force microscopy

详细信息    查看全文

• 作者：Andrea Freikamp^a ; Alexander Mehlich^b ; Christoph Klingner^a ; Carsten Grashoff^a ; ^{cgrasho@biochem.mpg.de}
• 关键词：FRET ; fluorescence resonance energy transfer ; GFP ; green fluorescent protein ; HP35 ; villin-headpiece peptide ; HP35st ; stable villin-headpiece peptide ; MTFM ; molecular tension&ndash ; based fluorescence microscopy ; MTS ; molecular tension sensor ; MUC-1 ; mucin-1 ; TSMod ; tension sensor module ; PECAM-1 ; platelet endothelial cell adhesion molecule-1 ; PEG ; polyethylene glycol ; pN ; piconewton ; TGT ; tension gauge tether
• 刊名：Journal of Structural Biology
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：197
• 期：1
• 页码：37-42
• 全文大小：1577 K
• 卷排序：197

文摘

The ability of cells to sense and respond to mechanical forces is crucial for a wide range of developmental and pathophysiological processes. The molecular mechanisms underlying cellular mechanotransduction, however, are largely unknown because suitable techniques to measure mechanical forces across individual molecules in cells have been missing. In this article, we highlight advances in the development of molecular force sensing techniques and discuss our recently expanded set of FRET-based tension sensors that allows the analysis of mechanical forces with piconewton sensitivity in cells. In addition, we provide a theoretical framework for the design of additional tension sensor modules with adjusted force sensitivity.