Reduction of d-lactate content in sauerkraut using starter cultures of recombinant Leuconostoc mesenteroides expressing the ldhL gene

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• 作者：Qing Jin¹ ; ^&Dagger ; ; Ling Li² ; ^&Dagger ; ; Jin Seok Moon² ; Seung Kee Cho² ; Yu Jin Kim³ ; Soo Jin Lee² ; Nam Soo Han² ; ^{namsoo@cbnu.ac.kr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Leuconostoc ; d-/l-Lactate ; d-/l-Lactate dehydrogenase ; Lactic acid bacteria ; Sauerkraut
• 刊名：Journal of Bioscience and Bioengineering
• 出版年：2016
• 出版时间：May 2016
• 年：2016
• 卷：121
• 期：5
• 页码：479-483
• 全文大小：386 K

文摘

The d-form of lactate, which causes metabolic stress upon excessive dietary intake, is mainly produced by Leuconostoc sp., the predominant species in sauerkraut. To shift the metabolic flux of d-lactate from pyruvate to l-lactate, we expressed the l-lactate dehydrogenase (ldhL) gene in Leuconostoc mesenteroides ATCC 8293. The ldhL gene from Lactobacillus plantarum was introduced into L. mesenteroides using the shuttle vectors pLeuCM and pLeuCM42. To elevate the expression level of ldhL in L. mesenteroides, the nucleotides for pyruvate kinase promoter were fused to ldhL and cloned into above vectors to construct pLC18pkL and pLC42pkL. As results, introduction of pLC42pkL in L. mesenteroides significantly improved both l-LDH activity and l-lactate productivity during fermentation, decreasing the d-/l-lactate ratio. When used as a starter culture for sauerkraut fermentation, recombinant L. mesenteroides harboring pLC42pkL increased l-lactate concentration and decreased d-lactate concentration compared to the wild type strain. We newly developed a recombinant L. mesenteroides which has high l-lactate dehydrogenase activity and applied this strain to minimize the harmful effect of d-lactate during the sauerkraut fermentation. To the best of our knowledge, we demonstrate for the first time the effective use of recombinant Leuconostoc sp. for quality improvement of fermented foods.