Pectinase-treated Panax ginseng ameliorates hydrogen peroxide-induced oxidative stress in GC-2 sperm cells and modulates testicular gene expression in aged rats

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• 作者：Spandana Rajendra Kopalli¹ ; Kyu-Min Cha¹ ; Min-Sik Jeong¹ ; Sang-Ho Lee¹ ; Jong-Hwan Sung² ; Seok-Kyo Seo³ ; Si-Kwan Kim¹ ; ^{skkim@kku.ac.kr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：oxidative enzymes ; Panax ginseng ; pectinase ; spermatogenesis ; subfertility
• 刊名：Journal of Ginseng Research
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：40
• 期：2
• 页码：185-195
• 全文大小：1554 K

文摘

To investigate the effect of pectinase-treated Panax ginseng (GINST) in cellular and male subfertility animal models.

Methods

Hydrogen peroxide (H₂O₂)-induced mouse spermatocyte GC-2spd cells were used as an in vitro model. Cell viability was measured using MTT assay. For the in vivo study, GINST (200 mg/kg) mixed with a regular pellet diet was administered orally for 4 mo, and the changes in the mRNA and protein expression level of antioxidative and spermatogenic genes in young and aged control rats were compared using real-time reverse transcription polymerase chain reaction and western blotting.

Results

GINST treatment (50 μg/mL, 100 μg/mL, and 200 μg/mL) significantly (p < 0.05) inhibited the H₂O₂-induced (200 μM) cytotoxicity in GC-2spd cells. Furthermore, GINST (50 μg/mL and 100 μg/mL) significantly (p < 0.05) ameliorated the H₂O₂-induced decrease in the expression level of antioxidant enzymes (peroxiredoxin 3 and 4, glutathione S-transferase m5, and glutathione peroxidase 4), spermatogenesis-related protein such as inhibin-α, and specific sex hormone receptors (androgen receptor, luteinizing hormone receptor, and follicle-stimulating hormone receptor) in GC-2spd cells. Similarly, the altered expression level of the above mentioned genes and of spermatogenesis-related nectin-2 and cAMP response element-binding protein in aged rat testes was ameliorated with GINST (200 mg/kg) treatment. Taken together, GINST attenuated H₂O₂-induced oxidative stress in GC-2 cells and modulated the expression of antioxidant-related genes and of spermatogenic-related proteins and sex hormone receptors in aged rats.

Conclusion

GINST may be a potential natural agent for the protection against or treatment of oxidative stress-induced male subfertility and aging-induced male subfertility.