- 作者：H.-D. Zhao ; W.-G. Zhang ; M.-N. Sun ; Q.-F. Duan ; F.-L. Li ; H. Li
- 关键词：Trophoblast ; SEPSECS ; PG ; hCG ; Apoptosis ; Proliferation
- 刊名：Placenta
- 出版年：November, 2013
- 年：2013
- 卷：34
- 期：11
- 页码：967-972
- 全文大小：1305 K
Objectives
To investigate whether Sep (O-phosphoserine) tRNA: Sec (selenocysteine) synthase (SEPSECS), which plays an essential role in the synthesis of selenoprotein, affects proliferation, apoptosis and hormone secretion of human trophoblast cells.Methods
Human trophoblast JEG-3 cells were divided into four groups: control group, SEPSECS silenced-expression group, empty vector group and SEPSECS over-expression group. Over-expression and silenced-expression were achieved by transfection with plasmid DNA or RNA oligonucleotide, respectively. 3-[4,5-dimethylthiazol-2-yl] -2,5-diphenyltetrazolium bromide (MTT) and colony formation assays were performed to investigate cell proliferation, while apoptosis was tested by annexin V-FITC, PI double staining and caspases-3 activation assays, enzyme-linked immunosorbent assay (ELISA) was used to determine the level of progesterone (PG) and human chorionic gonadotropin (hCG).
Results
SEPSECS silenced-expression clearly inhibited proliferation of JEG-3 cells (p聽<聽0.05), significantly induced cell apoptosis (p聽<聽0.01) and reduced the production of PG and hCG (p聽<聽0.05). On the contrary, SEPSECS over-expression significantly promoted both cell proliferation (p聽<聽0.01) and secretion of PG and hCG (p聽<聽0.05).
Conclusions
SEPSECS significantly affects proliferation, apoptosis and hormone secretion of human trophoblast cells, suggesting that a potential relationship exists among SEPSECS, cell proliferation, apoptosis and hormone production of human placental trophoblast cells. Furthermore, this may provide a clue to uncover the relationship between selenium and human placental in association with an emphasis on the importance of selenium adequacy during pregnancy.