Development of CYB5-fusion monitoring system for efficient periplasmic expression of multimeric proteins in Escherichia coli
详细信息 查看全文
- 作者：Dmitri Dormeshkin ; Andrei Gilep ; agilep@iboch.bas-net.by" class="auth_mail" title="E-mail the corresponding author ; Gennady Sergeev ; Sergey Usanov
- 关键词：Recombinant antibodies ; Cytochrome b5 ; Protein expression monitoring ; Protein quantification
- 刊名：Protein Expression and Purification
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：128
- 期：Complete
- 页码：60-66
- 全文大小：869 K
文摘
- ass="listitem" id="list_ulist0010">
- ass="label">•
CYB5-fusion periplasm system for efficient expression of multimeric proteins was developed.
- ass="label">•
scFv and Fab antibodies were expressed and characterized utilizing this system.
- ass="label">•
CYB5 enhance periplasmic expression of antibody fragments.
- ass="label">•
CYB5 does not interfere with antibodies affinity and specificity.
- ass="label">•
Different spectrum measurement allows us to quantify antibodies in turbid solutions.