Interaction in vivo
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- 作者：Elvira R. Eivazova ; Aleksey Gavrilov ; Iryna Pirozhkova ; Andrei Petrov ; Olga V. Iarovaia ; Sergey V. Razin ; Marc Lipinski ; Yegor S. Vassetzky
- 关键词：Ifng gene domain ; chromatin conformation capture ; nuclear matrix ; recombination ; topoisomerase II
- 刊名：Journal of Molecular Biology
- 出版年：2009
- 出版时间：6 March 2009
- 年：2009
- 卷：386
- 期：4
- 页码：929-937
- 全文大小：424 K
文摘
In interphase nuclei as in metaphase chromosomes, the genome is organized into topologically closed loop domains. Here, we have mapped the ends of the loop domain that contains the Ifng (interferon-γ) gene in primary and cultured murine T-lymphocytes. To determine whether the ends of the loop are located in close proximity to each other in the nuclear space, the 3C (chromosome conformation capture) technique, which detects protein-mediated DNA–DNA interactions, was utilized. A strong interaction was demonstrated between the two ends of the loop, which were close enough to become cross-linked in vivo in the presence of paraformaldehyde. Chromatin immunoprecipitation combined with the 3C technique demonstrated that topoisomerase IIα and MeCP2, but not topoisomerase IIβ, heterochromatin-associated protein HP1 or CTCF, were involved in this interaction. The present findings have important implications in terms of mechanisms of illegitimate recombination that can result in chromosomal translocations and deletions.