Enhancement of fructose utilization from sucrose in the cell for improved l-serine production in engineered Corynebacterium glutamicum

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• 作者：Xiaomei Zhang^a ; ^b ; Liping Yao^a ; ^b ; Guoqiang Xu^a ; ^b ; ^c ; Jiafen Zhu^a ; ^b ; Xiaojuan Zhang^a ; ^c ; Jinsong Shi^a ; ^b ; Zhenghong Xu^a ; ^b ; ^c ; ^{zhenghxu@jiangnan.edu.cn}
• 关键词：l-Serine ; Corynebacterium glutamicum ; Fructose ; scrK ; pfkA
• 刊名：Biochemical Engineering Journal
• 出版年：2017
• 出版时间：15 February 2017
• 年：2017
• 卷：118
• 期：Complete
• 页码：113-122
• 全文大小：2161 K
• 卷排序：118

文摘

Direct utilization of fructose from sucrose in the cell was investigated. Heterologous expression of scrK in C. glutamicum improved fructose consumption. Co-expression of pfkA and scrK aided fructose consumption and l-serine accumulation. The engineered C. glutamicum strain produced 30.6 g/L l-serine in a 5-L bioreactor. After two feedings of sucrose, the l-serine production increased up to 37.0 g/L.