Effects of β-AP peptides on activation of the transcription factor NF-κB and in cell proliferation in glial cell cultures

详细信息    查看全文

• 作者：Casal ; Carme ; Serratosa ; Joan ; Tusell ; Josep M.
• 关键词：β ; -Amyloid ; Peptide aggregation ; NF-κ ; B Transcription factor activation ; Glial activation ; Glial proliferation
• 刊名：Neuroscience Research
• 出版年：2004
• 出版时间：March, 2004
• 年：2004
• 卷：48
• 期：3
• 页码：315-323
• 全文大小：779 K

文摘

The effect of two beta amyloid peptides (Aβ 25/35 and Aβ 1/42) on the activation of the transcription factor kappaB (NF-κB) in pure astroglial, pure microglial and mixed glial cell cultures was compared by means of single or double immunofluorescence and Western blot techniques. We also studied the effect of both peptides in cell proliferation in mixed glial cultures and pure astrocytes. The Aβ 1/42 peptide induced the activation of NF-κB in all studied cell cultures and its effect was potentiated by interferon-gamma (IFN-γ). Aβ 25/35 alone did not induce NF-κB activation, but Aβ 25/35 plus IFN-γ induced the activation of the transcription factor in the mixed and pure microglial cultures, although not in pure astroglia. The Aβ 1/42 peptide, but not Aβ 25/35, induced proliferation in pure astroglial and mixed glial cell cultures. The results suggest that the state of peptide aggregation is related to their ability to activate glial cells.