A multi-tolerant low molecular weight mannanase from Bacillus sp. CSB39 and its compatibility as an industrial biocatalyst
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- 作者：Sudip Regmia ; 1 ; Pradeep G.C.a ; 1 ; Yun Hee Choia ; Yoon Seok Choia ; Ji Eun Choia ; Seung Sik Chob ; Jin Cheol Yooa ; jcyu@chosun.ac.kr" class="auth_mail" title="E-mail the corresponding author
- 关键词：Bacillus sp. ; Biocatalyst ; Biodegradation ; Galactomannan ; Mannanase
- 刊名：Enzyme and Microbial Technology
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：92
- 期：Complete
- 页码：76-85
- 全文大小：1457 K
文摘
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Surfactant-, NaCl-, urea-, and protease-tolerant mannanase was purified and characterized.
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MnCSB39 had the lowest molecular mass of ∼30 kDa from Bacillus sp.
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Optimal pH and temperature for enzymatic activity were 7.5 and 70 °C respectively.
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The Km and Vmax values were 0.0819 mg/mL and 1099 ± 1.0 Umg−1, respectively.
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Thermodynamics also support its hydrolytic efficiency, feasibility and spontaneity.