A direct RP-HPLC method for the determination of furanic aldehydes and acids in honey

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• 作者：Nadia Spano ; Marco Ciulu ; Ignazio Floris ; Angelo Panzanelli ; Maria I. Pilo ; Paola C. Piu ; Severyn Salis ; Gavino Sanna
• 关键词：Honey ; 5-Hydroxymethyl-2-furaldehyde (HMF) ; Furfural ; 2-Furaldehyde ; 3-Furaldehyde ; 2-Furoic acid ; 3-Furoic acid
• 刊名：Talanta
• 出版年：2009
• 出版时间：15 April 2009
• 年：2009
• 卷：78
• 期：1
• 页码：310-314
• 全文大小：296 K

文摘

In this study 5-hydroxymethyl-2-furaldehyde (HMF), 2-furaldehyde, 3-furaldehyde, 2-furoic acid and 3-furoic acid are contemporarily determined in honey using a swift and direct RP-HPLC approach. The validation protocol was performed in terms of detection and quantification limits, precision (by repeatability and reproducibility), linearity and accuracy (by recovery tests); the acceptability of the precision and accuracy results was positively verified using Horwitz's model and AOAC guidelines, respectively. The method was tested on 18 honey samples of different ages, and botanical and geographical origin. HMF and 2-furaldehyde correlated highly with the age of the samples, whereas no correlation was observed with regards to 2-furaldehyde and 2-furoic acid. Hypotheses relating to the formation of minority furanic compounds are also proposed.