Purification and efficient refolding process for recombinant tissue-type plasminogen activator derivative (reteplase) using glycerol and Tranexamic acid

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• 作者：Seyed Babak Mousavi^a ; Ahmad Fazeli^c ; Seyed Abbas Shojaosadati^a ; ^{shoja_sa@modares.ac.ir} ; Mohammad Reza Fazeli^b ; Sameereh Hashemi-Najafabadi^a
• 关键词：Tranexamic acid (PubChem CID ; 5526) ; Glycerol (PubChem CID ; 753) ; l-Arginine (PubChem CID ; 6322) ; l-Lysine (PubChem CID ; 5962) ; Tween-80 (PubChem CID ; 5281955) ; Ethylene Glycol (PubChem CID ; 174) ; Urea (PubChem CID ; 1176) ; Glycine (PubChem CID ; 750) ; Triton-X100 (PubChem CID ; 5590) ; Sucrose (PubChem CID ; 5988)
• 刊名：Process Biochemistry
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：53
• 期：Complete
• 页码：135-144
• 全文大小：2093 K
• 卷排序：53

文摘

In the present study, Txa was used as a refolding aid for the first time. The synergic effect of urea, glycerol and Txa effectively increased the refolding yield of reteplase. Txa increases the solubility of the folding intermediates by stabilizing the conformation, which results in increased refolding yield.