Disposable orbitally shaken TubeSpin bioreactor 600 for Sf9 cell cultivation in suspension

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• 作者：Dominique T. Monteil^a ; ¹ ; Xiao Shen^a ; ¹ ; ² ; Giulia Tontodonati^a ; ³ ; Lucia Baldi^a ; David L. Hacker^a ; ^b ; Florian M. Wurm^a ; ² ; ^{florian.wurm@exellgene.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Orbital shaking ; Disposable bioreactor ; Sf9 cells ; Cell growth ; Gas transfer
• 刊名：Analytical Biochemistry
• 出版年：2016
• 出版时间：15 July 2016
• 年：2016
• 卷：505
• 期：Complete
• 页码：26-28
• 全文大小：452 K

文摘

Disposable orbitally shaken TubeSpin bioreactor 600 tubes (TS600s) were recently developed for the bench-scale cultivation of animal cells in suspension. Here we compared batch cultures of Sf9 insect cells in TS600s, spinner flasks, and shake flasks. Superior cell growth was observed in TS600s and shake flasks as compared with spinner flasks, and more favorable oxygen-enriched cell culture conditions were observed in TS600s as compared with either spinner or shake flasks. The results demonstrated the suitability of TS600s as a disposable vessel for the cultivation of Sf9 cells in suspension.