Intersectin 1 forms complexes with SGIP1 and Reps1 in clathrin-coated pits
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- 作者：Oleks ; r Dergai ; Olga Novokhatska ; Mykola Dergai ; Inessa Skrypkina ; Liudmyla Tsyba ; Jacques Moreau ; Alla Rynditch
- 关键词：Endocytosis ; Adaptor proteins ; Intersectin 1 ; Protein interactions ; SGIP1 ; Reps1
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2010
- 出版时间：12 November 2010
- 年：2010
- 卷：402
- 期：2
- 页码：408-413
- 全文大小：773 K
文摘
Intersectin 1 (ITSN1) is an evolutionarily conserved adaptor protein involved in clathrin-mediated endocytosis, cellular signaling and cytoskeleton rearrangement. ITSN1 gene is located on human chromosome 21 in Down syndrome critical region. Several studies confirmed role of ITSN1 in Down syndrome phenotype. Here we report the identification of novel interconnections in the interaction network of this endocytic adaptor. We show that the membrane-deforming protein SGIP1 (Src homology 3-domain growth factor receptor-bound 2-like (endophilin) interacting protein 1) and the signaling adaptor Reps1 (RalBP associated Eps15-homology domain protein) interact with ITSN1 in vivo. Both interactions are mediated by the SH3 domains of ITSN1 and proline-rich motifs of protein partners. Moreover complexes comprising SGIP1, Reps1 and ITSN1 have been identified. We also identified new interactions between SGIP1, Reps1 and the BAR (Bin/amphiphysin/Rvs) domain-containing protein amphiphysin 1. Immunofluorescent data have demonstrated colocalization of ITSN1 with the newly identified protein partners in clathrin-coated pits. These findings expand the role of ITSN1 as a scaffolding molecule bringing together components of endocytic complexes.