Tandem repeat knockout utilizing the CRISPR/Cas9 system in human cells
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- 作者：Qingyan Lv1 ; Liangxue Lai1 ; Lin Yuan ; Yuning Song ; Tingting Sui ; Zhanjun Li ; lizj_1998@jlu.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：sgRNA ; Single guide RNA ; Humanized Cas9 ; hCas9 ; Humanized S. pyogenes ; hSpCas9 ; Double-strand DNA breaks ; DSBs ; Tandem repeat units ; TRs ; Potential off-target sites located in the genome ; POTS ; Off-target sites located in the TRs ; OTS ; Protospacer adjacent motif ; PAM
- 刊名：Gene
- 出版年：2016
- 出版时间：15 May 2016
- 年：2016
- 卷：582
- 期：2
- 页码：122-127
- 全文大小：1451 K
文摘
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CRISPR/Cas9-mediated tandem repeat knockout was proposed.
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Deletions up to 330 bp were demonstrated in the tandem repeats of MAGEL2.
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Off-target effects were identified for only one of the two sgRNAs used.