Insert, remove or replace: A highly advanced genome editing system using CRISPR/Cas9
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- 作者：S. Antony Ceasara ; b ; 1 ; Vinothkumar Rajanc ; 1 ; Sergey V. Prykhozhijd ; 1 ; Jason N. Bermanc ; d ; Jason.berman@iwk.nshealth.ca" class="auth_mail" title="E-mail the corresponding author ; S. Ignacimuthua ; e ; eriloyola@hotmail.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Genome editing ; CRISPR ; Cas9 ; Mutation ; Indel ; sgRNA ; Double stranded breaks
- 刊名：Biochimica et Biophysica Acta (BBA)-Molecular Cell Research
- 出版年：2016
- 出版时间：September 2016
- 年：2016
- 卷：1863
- 期：9
- 页码：2333-2344
- 全文大小：871 K
文摘
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CRISPR/Cas9 system has emerged as the most popular tool for genome editing.
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An sgRNA guides Cas9 nuclease to cause double-stranded breaks in DNA.
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CRISPR/Cas9 system has been applied in diverse organisms including human cells for the precise alterations of genome.
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Several online tools are available for the designing of sgRNA and to predict off-target effects.