- 作者：Arash Khojasteh ; DMD ; MS? ; arashkhojasteh@yahoo.com ; Abbas Khodayari ; DMD ; MS&dagger ; ; Farzaneh Rahimi ; MD ; MS&Dagger ; ; Mohamad Hossain Ghaderian ; MD ; PhD ; MS§ ; ; Mohamad Jafarian ; DMD ; MS¡Î ; Alireza Nayebi ; DMD ; MS¶ ; ; Reza Akbarzadeh Najar ; MSc# ; Akram Tabatabayipanah ; PhD?? ; Alireza Jahangirnia ; DMD&dagger ; &dagger ;
- 刊名：Journal of Oral and Maxillofacial Surgery
- 出版年：2013
- 出版时间：January, 2013
- 年：2013
- 卷：71
- 期：1
- 页码：62-65
- 全文大小：365 K
Purpose
The high rate of p16 gene alterations in malignant neoplasms suggests the important effect of this tumor-suppressor gene mutation on the malignant behavior of tumoral lesions. The present study investigated the possible methylation of the p16 tumor in ameloblastic carcinoma, ameloblastoma, and dental follicles.Materials and Methods
Eighteen samples of ameloblastic carcinoma, ameloblastoma, and dental follicles of mandibular impacted third molar were selected from available documents in the archives of the Department of Oral and Maxillofacial Pathology, Taleghani Hospital and the Department of Oral and Maxillofacial Pathology, Shahid Beheshti University of Medical Sciences, Tehran, Iran. After confirming the initial diagnosis, 6-¦Ìm sections were used for DNA extraction. A CpG island methylation of p16 was identified by polymerase chain reaction.
Results
Although CpG methylation of p16 was observed in all ameloblastic carcinoma samples, only 1 ameloblastoma specimen exhibited the mutation. The mutation was not detected in other ameloblastoma specimens or in any dental follicle sample.
Conclusions
The p16 alteration might play a role in the malignant progression of ameloblastic carcinoma. It is worth mentioning that ameloblastoma can be further differentiated from ameloblastic carcinoma based on molecular observations.