Evaluation of two commercial real-time PCR kits for detection of pandemic (H1N1) 2009 virus in Beijing

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• 作者：Guilan Lu^a ; ^{2000shanda@163.com} ; Hanqiu Yan^a ; ^{hqyan907@sina.com} ; Yaowu Yang^b ; ^c ; ^{smallyyw@163.com} ; Shujuan Cui^a ; ^{cuisj2003@126.com} ; Yanning Lü ; ^a ; ^{lvyanning@yahoo.com} ; Xin Zhang^a ; ^{zhangxin19751@163.com} ; Daitao Zhang^a ; ^{zhangdaitao@hotmail.com} ; Peng Yang^a ; ^{clairecaoyang@163.com} ; Fang Huang^a ; ^{hfxdd1775@yahoo.com.cn} ; Jianwei Wang^b ; ^{wangjw28@163.com} ; Quanyi Wang^a ; ^{bjcdcxm@126.com}
• 关键词：Evaluation ; Commercial PCR kits ; 2009 pandemic H1N1
• 刊名：Journal of Virological Methods
• 出版年：2013
• 出版时间：March, 2013
• 年：2013
• 卷：188
• 期：1-2
• 页码：25-28
• 全文大小：170 K

文摘

Active surveillance and diagnosis of the influenza pandemic (H1N1) 2009 (pH1N1) have played a critical role in the effective control and prevention of the pandemic in China. Although several commercially available real-time PCR kits for pH1N1 virus have been used in diagnostic laboratories in Beijing, little has been known about the performance of these kits for detecting pH1N1 virus.

In this study, the performance of two commercial real-time PCR kits in Beijing was evaluated. Analysis of clinical samples showed that the positive detection rate for the AgPath-ID? kit (38.2 % ) was significantly higher than that for the Da An H1N1 kit (30.0 % ) (McNemar's chi-square test, P = 0.000). The limit of detection (LOD) of the AgPath-ID? kit was 10², 10², and 10³ copies/reaction for the Influenza A (set 1), H1N1 Influenza A (set 2) and H1N1 Influenza A Sub H1 (set 3) genes, respectively, whereas the LOD of the Da An kit was 10³ copies/reaction for both H1 and N1 genes. Although the AgPath-ID? kit exhibited a significantly higher detection rate for pH1N1 than the Da An kit, cross-reactivity to A/PR8/34 was found for the AgPath-ID? kit for H1N1 Influenza A (set 2).