Determination of viable Salmonellae from potable and source water through PMA assisted qPCR

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• 作者：Gulshan Singh^a ; Poornima Vajpayee^a ; Saurabh Bhatti^a ; Nirmala Ronnie^b ; Nimish Shah^b ; Peter McClure^b ; Rishi Shanker^a ; ^{rishishanker1@rediffmail.com} ; ^{rishishanker@gmail.com} ; ^{rishi@iitr.res.in}
• 关键词：Viable salmonellae detection in low number ; Source and potable water ; Propidium monoazide assisted qPCR
• 刊名：Ecotoxicology and Environmental Safety
• 出版年：2013
• 出版时间：1 July, 2013
• 年：2013
• 卷：93
• 期：Complete
• 页码：121-127
• 全文大小：764 K

文摘

Resource constrained countries identified as endemic zones for pathogenicity of Salmonella bear an economic burden due to recurring expenditure on medical treatment. qPCR used for Salmonella detection could not discriminate between viable and nonviable cells. Propidium monoazide (PMA) that selectively penetrates nonviable cells to cross-link their DNA, was coupled with ttr gene specific qPCR for quantifying viable salmonellae in source/potable waters collected from a north Indian city. Source water (raw water for urban potable water supply) and urban potable water exhibited viable salmonellae in the range of 2.1¡Á10⁴?2.6¡Á10⁶ and 2-7160 CFU/100 mL, respectively. Potable water at water works exhibited DNA from dead cells but no viable cells were detected. PMA assisted qPCR could specifically detect low numbers of live salmonellae in Source and potable waters. This strategy can be used in surveillance of urban potable water distribution networks to map contamination points for better microbial risk management.