IFNγ primes macrophages for inflammatory activation by high molecular weight hyaluronan
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- 作者：Mark A. Wallet ; Shannon M. Wallet ; Giorgio Guiulfo ; John W. Sleasman ; Maureen M. Goodenow
- 关键词：Rheumatoid arthritis ; Inflammation ; Hyaluronan ; Macrophages ; Interferon gamma ; Interleukin-10 ; Interleukin-12 ; Tumor necrosis factor ; c-Jun
- 刊名：Cellular Immunology
- 出版年：2010
- 出版时间：2010
- 年：2010
- 卷：262
- 期：2
- 页码：84-88
- 全文大小：345 K
文摘
The objective was to assess outcomes of IFNγ-priming upon macrophage activation by the synovial macromolecule high molecular weight hyaluronan [HMW-HA] in the context of rheumatoid arthritis inflammation. Human macrophages primed by IFNγ and activated by HMW-HA were evaluated for cytokine secretion by ELISA and Milliplex assay and activation profiles by nuclear transcription factor EIA. IFNγ-primed, HMW-HA-activated macrophages produced elevated levels of TNF and secreted the TH1 cytokine IL-12p70, while IFNγ suppressed HMW-HA-induced secretion of the regulatory cytokine IL-10 and activation of the transcription factor c-Jun. IFNγ modulates the HMW-HA-induced cytokine response profile promoting macrophage activation and inflammatory TH1 cytokine secretion.