This study aimed to assess the ability of a Salmonella typhimurium-mediated Avain Reovirus DNA vaccine in eliciting antibody production. Six-day-old SPF chickens were orally immunized with SL7207 (pVAX-¦ÒC) twice at 2-week interval, detectable antibody was generated 2 weeks after immunization and was significantly higher than the control groups (P < 0.01) and ten chickens (66.7 % ) were considered safe in the subsequent challenge. These results show that SL7207 (pVAX-¦ÒC) can induce protective antibody in chickens and the newly-constructed vaccine is also effective in protection chickens against ARV infection.