A pseudotype baculovirus-mediated vaccine confers protective immunity against lethal challenge with H5N1 avian influenza virus in mice and chickens

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• 作者：Qunfeng Wu ; Liurong Fang ; Xuebao Wu ; Bin Li ; Rui Luo ; Zhengjun Yu ; Meilin Jin ; Huanchun Chen ; Shaobo Xiao
• 关键词：H5N1 avian influenza virus ; Pseudotype baculovirus ; Hemagglutinin protein ; Vaccine
• 刊名：Molecular Immunology
• 出版年：2009
• 出版时间：July 2009
• 年：2009
• 卷：46
• 期：11-12
• 页码：2210-2217
• 全文大小：3944 K

文摘

Baculovirus has emerged recently as a novel and attractive gene delivery vehicle for mammalian cells. In this study, baculovirus pseudotyped with vesicular stomatitis virus glycoprotein was used as a vector to express the hemagglutinin (HA) protein of highly pathogenic H5N1 avian influenza virus, A/Chicken/Hubei/327/2004 (HB/327). The resultant recombinant baculovirus (BV-G-HA) mediated gene delivery and HA expression efficiently in mammalian cells. Mice immunized with 1 × 10⁹ PFU of BV-G-HA developed significantly higher levels of H5-specific antibodies and cellular immunity than those that received 100 μg of DNA vaccines expressing HA, and were completely protected from lethal challenge with HB/327. Different vaccination doses were further tested in chickens, and these experiments demonstrated that 1 × 10⁸ PFU of BV-G-HA offered complete protection from challenge with 100 LD₅₀ of HB/327. These data indicate that the pseudotype baculovirus-mediated vaccine could be utilized as an alternative strategy against the pandemic spread of H5N1 influenza virus.