- 作者：Shaotang Zhou ; Arun P. Palanisamy ; John W. McGillicuddy ; Tom P. Theruvath ; Sukru H. Emre ; Kenneth D. Chavin
- 关键词：Mouse model ; Transplant ; Liver ; Obesity ; OLT ; Arterialized ; Anastomosis ; Stent
- 刊名：Journal of Surgical Research
- 出版年：March, 2014
- 年：2014
- 卷：187
- 期：1
- 页码：297-301
- 全文大小：834 K
Background
Arterialized orthotopic liver transplantation (OLT) in the mouse mimics human liver transplantation physiologically and clinically. The present method of sutured anastomosis for reconstruction of the hepatic artery is complex and is associated with high incidence of complications and failure. This makes the endpoint assessment of using this complex model difficult because of the many variables of the technical aspect.Methods
A total of 14 pairs of donors and recipients from syngeneic male mice were used for arterialized OLT. The grafts were stored in University of Wisconsin solution at 4掳C for less than 4聽h, and the recipients underwent OLT using a two-cuff technique. The arterial reconstruction was facilitated by the use of a single stent connecting the donor liver artery segment to the recipient common hepatic artery.
Results
All 14 recipients survived with the time for arterial reconstruction ranging from 4-10聽min. Patency of the artery was confirmed by transecting the artery near the graft 2 and 14聽d after transplantation. At day 2, five of the six arteries transected were patent and at day 14, seven of the remaining eight were patent for an overall patency rate of 85.7%.
Conclusions
The stent-facilitated arterial reconstruction can be done quickly with a high patency rate. This model expands the translational research efforts to address marginal livers such as steatotic livers.