Suppression of UVA-mediated release of labile iron by epicatechin—A link to lysosomal protection

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• 作者：Sharmila Basu-Modak ; Dalia Ali ; Matt Gordon ; Tobias Polte ; Anthie Yiakouvaki ; Charareh Pourzand ; Catherine Rice-Evans and Rex M. Tyrrell
• 关键词：Catechin ; UVA ; Iron ; Protection ; Lysosome ; Free radicals
• 刊名：Free Radical Biology and Medicine
• 出版年：2006
• 出版时间：15 October 2006
• 年：2006
• 卷：41
• 期：8
• 页码：1197-1204
• 全文大小：420 K

文摘

UVA (320–380 nm) radiation generates an oxidative stress in cells and leads to an immediate release of potentially damaging labile iron pools in human skin cells. Treatment of cultured skin fibroblasts for several hours with physiologically relevant concentrations of either epicatechin (EC), a flavonoid plant constituent present in foods, or methylated epicatechin (3′-O-methyl epicatechin, MeOEC), its major human metabolite, prevents this iron release. The similarity of the effectiveness of EC and MeOEC argues against chelation as the mechanism of iron removal. Evidence based on measurements of lysosomal integrity strongly supports the hypothesis that the catechins protect against lysosomal destruction by UVA. Such damage would normally lead to protease release, which has been previously shown to cause ferritin degradation and release of labile iron.