Cancer cells incorporate and remodel exogenous palmitate into structural and oncogenic signaling lipids

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• 作者：Sharon M. Louie ; Lindsay S. Roberts ; Melinda M. Mulvihill ; Kunxin Luo ; Daniel K. Nomura
• 关键词：FFA ; Free fatty acid ; QQQ-LC/MS ; triple quadrupole liquid chromatography-mass spectrometry ; MAGL ; monoacylglycerol lipase ; PEG ; polyethylene glycol ; EMT ; epithelial-to-mesenchymal transition ; SRM ; single reaction monitoring ; AC ; acyl carnitines ; PC ; phosp
• 刊名：Biochimica et Biophysica Acta (BBA)/Molecular and Cell Biology of Lipids
• 出版年：2013
• 出版时间：October, 2013
• 年：2013
• 卷：1831
• 期：10
• 页码：1566-1572
• 全文大小：1023 K

文摘

m>De novom> lipogenesis is considered the primary source of fatty acids for lipid synthesis in cancer cells, even in the presence of exogenous fatty acids. Here, we have used an isotopic fatty acid labeling strategy coupled with metabolomic profiling platforms to comprehensively map palmitic acid incorporation into complex lipids in cancer cells. We show that cancer cells and tumors robustly incorporate and remodel exogenous palmitate into structural and oncogenic glycerophospholipids, sphingolipids, and ether lipids. We also find that fatty acid incorporation into oxidative pathways is reduced in aggressive human cancer cells, and instead shunted into pathways for generating structural and signaling lipids. Our results demonstrate that cancer cells do not solely rely on m>de novom> lipogenesis, but also utilize exogenous fatty acids for generating lipids required for proliferation and protumorigenic lipid signaling. This article is part of a special issue entitled Lipid Metabolism in Cancer.