Effect of simulated transport stress on the rat small intestine: A morphological and gene expression study

详细信息    查看全文

• 作者：Changrong Wan^a ; Peng Yin^a ; Xiaolong Xu^a ; Mingjiang Liu^a ; Shasha He^a ; Shixiu Song^a ; Fenghua Liu^b ; ^{liufenghua1209@126.com" class="auth_mail} ; Jianqin Xu^a ; ^{xujianqincau@126.com" class="auth_mail}
• 关键词：Transport stress ; Intestinal damage ; Microarray analysis ; Morphological study
• 刊名：Research in Veterinary Science
• 出版年：April, 2014
• 年：2014
• 卷：96
• 期：2
• 页码：355-364
• 全文大小：2942 K

文摘

The present study investigated the effects of simulated transport stress on morphology and gene expression in the small intestine of laboratory rats. Sprague Dawley rats were subjected to 35 掳C and 0.1脳g on a constant temperature shaker for physiological, biochemical, morphological and microarray analysis before and after treatment. The treatment induced obvious stress responses with significant decreases in body weight (P < 0.01), increases in rectal temperature, serum corticosterone (CORT), serum glucose (GLU), creatine kinase (CK) and lactate dehydrogenase (LDH) levels (P < 0.01), as well as expression of Hsp27/70/90 mRNA (P < 0.05; P < 0.01). The rat jejunum was severely damaged and apoptotic after mimicking transport stress, which may mainly be related to cell death, oxidation reduction and hormone imbalance determined by microarray analysis. The bioinformatics analysis from the present study would provide insight into the potential mechanisms underlying transport stress-induced injury in the rat small intestine.