Tritylisation of pyrene, perylene and coronene: a new family of switchable fluorescent labels
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- 作者：Shchepinov ; Mikhail S. ; Korshun ; Vladimir A. ; Egeland ; Ryan D. ; Southern ; Edwin M.
- 关键词：polycyclic aromatic compounds ; Friedel&ndash ; Crafts reactions ; Grignard reactions ; fluorescence ; nucleic acids
- 刊名：Tetrahedron Letters
- 出版年：2000
- 出版时间：June 19, 2000
- 年：2000
- 卷：41
- 期：25
- 页码：4943-4948
- 全文大小：302 K
文摘
The synthesis of novel fluorescent labels based on pyrene, perylene and coronene is described. Due to the trityl-type structure, their fluorescence may be reversibly switched on and off by changing the pH. This property can be used to expand the palette of fluorophores available for multicolour DNA detection on DNA chips. Some FRET and surface chemistry applications are also demonstrated.