Isotopic reinforcement of essential polyunsaturated fatty acids diminishes nigrostriatal degeneration in a mouse model of Parkinson's disease

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• 作者：Mikhail S. Shchepinov^a ;   ; ^{misha@retrotope.com} ; Vivian P. Chou^b ;   ; ^e ;   ; ^{vivian.chou@sri.com} ; Erik Pollock^c ;   ; ^{epolloc@uark.edu} ; J. William Langston^b ;   ; ^{jwlangston@thepi.org} ; Charles R. Cantor^a ;   ; ^d ;   ; ^{ccantor@scripps.edu} ; Robert J. Molinari^a ;   ; ^{bob@retrotope.com} ; Amy B. Manning-Boğ ; ^b ;   ; ^e ;   ; ^{amy.manningbog@sri.com}
• 关键词：Parkinson's disease ; Deuterium ; Mouse model ; Oxidative injury ; Polyunsaturated fatty acid ; Nigrostriatal pathway
• 刊名：Toxicology Letters
• 出版年：2011
• 出版时间：30 November 2011
• 年：2011
• 卷：207
• 期：2
• 页码：97-103
• 全文大小：791 K

文摘

Oxidative damage of membrane polyunsaturated fatty acids (PUFA) is thought to play a major role in mitochondrial dysfunction related to Parkinson's disease (PD). The toxic products formed by PUFA oxidation inflict further damage on cellular components and contribute to neuronal degeneration. Here, we tested the hypothesis that isotopic reinforcement, by deuteration of the bisallylic sites most susceptible to oxidation in PUFA may provide at least partial protection against nigrostriatal injury in a mouse model of oxidative stress and cell death, the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) model. Mice were fed a fat-free diet supplemented with saturated acids, oleic acid and essential PUFA: either normal, hydrogenated linoleic (LA, 18:2n ?6) and ¦Á-linolenic (ALA, 18:3n ?3) or deuterated 11,11-D2-LA and 11,11,14,14-D4-ALA in a ratio of 1:1 (to a total of 10 % mass fat) for 6 days; each group was divided into two cohorts receiving either MPTP or saline and then continued on respective diets for 6 days. Brain homogenates from mice receiving deuterated PUFA (D-PUFA) vs. hydrogenated PUFA (H-PUFA) demonstrated a significant incorporation of deuterium as measured by isotope ratio mass-spectrometry. Following MPTP exposure, mice fed H-PUFA revealed 78.7 % striatal dopamine (DA) depletion compared to a 46.8 % reduction in the D-PUFA cohort (as compared to their respective saline-treated controls), indicating a significant improvement in DA concentration with D-PUFA. Similarly, higher levels of the DA metabolite 3,4-dihydroxyphenylacetic acid (DOPAC) were detected in MPTP-exposure mice administered D-PUFA; however, saline-treated mice revealed no change in DA or DOPAC levels. Western blot analyses of tyrosine hydroxylase (TH) confirmed neuroprotection with D-PUFA, as striatal homogenates showed higher levels of TH immunoreactivity in D-PUFA (88.5 % control) vs. H-PUFA (50.4 % control) in the MPTP-treated cohorts. In the substantia nigra, a significant improvement was noted in the number of nigral dopaminergic neurons following MPTP exposure in the D-PUFA (79.5 % control) vs. H-PUFA (58.8 % control) mice using unbiased stereological cell counting. Taken together, these findings indicate that dietary isotopic reinforcement with D-PUFA partially protects against nigrostriatal damage from oxidative injury elicited by MPTP in mice.