Analysis of the evolutionarily conserved repeat motifs in the genome of the highly endangered central Indian swamp deer Cervus duvauceli branderi

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• 作者：Ali ; Sher ; Ansari ; Shehnaz ; Z. Ehtesham ; Nasreen ; Azfer ; Md. Asim ; Homkar ; Uday ; Gopal ; Rajesh ; et. al.
• 关键词：Barasingha (swamp deer) ; AP-PCR amplification ; Biodiversity ; Endangered species ; Genome analysis ; Molecular evolution ; AP-PCR ; arbitrarily primed polymerase chain reaction ; bp ; base pair(s) ; C. ; Cervus ; oligo ; oligodeoxyribonucleotide(s) ; u ; unit(s) ; UV
• 刊名：Gene
• 出版年：1998
• 出版时间：November 26, 1998
• 年：1998
• 卷：223
• 期：1-2
• 页码：361-367
• 全文大小：232 K

文摘

We have analyzed the genome of central Indian swamp deer Cervus duvauceli branderi, an inhabitant of the Kanha National Park, a wildlife conservatory in Central India, with a view to provide a genetic basis for their extinction. Evolutionarily conserved repeat sequence motifs (GATA)_3.75, TA(GATA)₄, (GACA)_3.75, (TGG)₆ and a set of mouse β-actin primers were used to uncover the sequence variation within and between related species by employing techniques of hybridization and AP-PCR amplification. The oligo probe carrying the GACA and TGG repeat motifs was found to be positive with Cervus genome, whereas (GATA)_3.75, TA(GATA)₄ and β-actin probes did not cross-hybridize with the same. AP-PCR amplification with (GACA)_3.75, unlike the (TGG)₆ primer, generated distinct bands in the range of 0.37–2.10 kb amongst different genomes including Cervus. A comparative genome analysis of other species using the AP-PCR approach with (GACA)_3.75 primer revealed the phylogenetic status of Cervus duvauceli branderi. From the analysis of a very limited number of Cervus DNA samples, we observed a high level of genetic homogeneity that may be a prime reason for the extinction of this species. This study has implications in the context of conservation of this endangered Cervus duvauceli branderi species.