Nanopartaicles were prepared between NF-κB/p65 antisense gene (NAG) and chitosan.
NAG chitosan NPs had low toxic effect on RAW264.7 macrophages.
NAG chitosan NPs were capable to deliver NAG into cytoplasm and nucleus.
NAG chitosan NPs significantly decreased the activation and protein expression level of NF-κB/p65.
Release level of inflammatory factors was decreased by NAG chitosan NPs.