Cloning and high level expression of the biologically active extracellular domain of Macaca mulatta CD40 in Pichia pastoris
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- 作者：Shengyun Zhu1 ; 2 ; Lin Wan1 ; Hao Yang ; Jingqiu Cheng ; Xiaofeng Lu ; xiaofenglu@yahoo.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：CD40 ; Chronic inflammatory disease ; Immunosuppression ; Rhesus monkey ; Pichia pastoris
- 刊名：Protein Expression and Purification
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：119
- 期：Complete
- 页码：19-26
- 全文大小：1251 K
文摘
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mmCD40Ig was expressed in Pichia pastoris at a high level of 15–20 mg/L.
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mmCD40Ig was glycosylated, dimerized and can specifically bind to CD40L.
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mmCD40Ig showed immunosuppressive activity in vitro.