Co-evolution of the archaeal tRNA-dependent amidotransferase GatCAB with tRNA^Asn

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• 作者：Suk Namgoong ; Kelly Sheppard ; R. Lynn Sherrer ; Dieter Sö ; ll
• 关键词：tRNA ; Transamidation ; Asn-tRNA ; Gln-tRNA ; Evolution
• 刊名：FEBS Letters
• 出版年：2007
• 出版时间：23 January 2007
• 年：2007
• 卷：581
• 期：2
• 页码：309-314
• 全文大小：328 K

文摘

The important identity elements in tRNA^Gln and tRNA^Asn for bacterial GatCAB and in tRNA^Gln for archaeal GatDE are the D-loop and the first base pair of the acceptor stem. Here we show that Methanothermobacter thermautotrophicus GatCAB, the archaeal enzyme, is different as it discriminates Asp-tRNA^Asp and Asp-tRNA^Asn by use of U49, the D-loop and to a lesser extent the variable loop. Since archaea possess the tRNA^Gln-specific amidotransferase GatDE, the archaeal GatCAB enzyme evolved to recognize different elements in tRNA^Asn than those recognized by GatDE or by the bacterial GatCAB enzyme in their tRNA substrates.