High resolution imaging using scanning ion conductance microscopy with improved distance feedback control

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• 作者：Chao Li ; Nicholas Johnson ; Victor Ostanin ; Andrew Shevchuk ; Liming Ying ; Yuri Korchev ; David Klenerman
• 关键词：Living biological cells ; Scanning ion conductance microscopy ; Distance feedback control
• 刊名：Progress in Natural Science
• 出版年：2008
• 出版时间：10 June 2008
• 年：2008
• 卷：18
• 期：6
• 页码：671-677
• 全文大小：935 K

文摘

Microscopy is an essential technique for observation on living cells. There is currently great interest in applying scanning probe microscopy to image-living biological cells in their natural environment at the nanometer scale. Scanning ion conductance microscopy is a new form of scanning probe microscopy, which enables non-contact high-resolution imaging of living biological cells. Based on a scanned nanopipette in physiological buffer, the distance feedback control uses the ion current to control the distance between the pipette tip and the sample surface. However, this feedback control has difficulties over slopes on convoluted cell surfaces, which limits its resolution. In this study, we present an improved form of feedback control that removes the contribution of up to the third-order slope from the ion current signal, hence providing a more accurate signal for controlling the distance. We show that this allows faster and lower noise topographic high-resolution imaging.