Effect of the sequence context of the AUG initiation codon on the rate of translation in dicotyledonous and monocotyledonous plant cells

详细信息    查看全文

• 作者：Tadatoshi  ; Sugio¹ ; Hideyuki  ; Matsuura¹ ; Takeshi  ; Matsui¹ ; Manabu  ; Matsunaga¹ ; Tatsuya  ; Nosho² ; Shigehiko  ; Kanaya² ; Atsuhiko  ; Shinmyo¹ ; Ko  ; Kato¹ ;   ; ^{kou@bs.naist.jp}
• 关键词：Translation efficiency ; Context around initiation codon ; Arabidopsis thaliana T87 ; Oryza sativa ; Protoplast
• 刊名：Journal of Bioscience and Bioengineering
• 出版年：2010
• 出版时间：February 2010
• 年：2010
• 卷：109
• 期：2
• 页码：170-173
• 全文大小：484 K

文摘

The sequence context around the AUG initiation codon strongly contributes to the translation initiation step in mammalian and plant cells. Here, we investigated the effect of the three nucleotides immediately upstream of the initiating AUG (positions ?3 to ?1) on the translation efficiency of a reporter gene, ¦Â-glucuronidase, in dicotyledonous and monocotyledonous plant cells.