A transfection reporter for the prevention of false-negative results in molecular beacon experiments

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• 作者：Tatsuya Toga ; Isao Kuraoka ; Akira Yasui ; Shigenori Iwai
• 关键词：Molecular beacon ; Base excision repair ; Phosphorothioate ; Thymine glycol
• 刊名：Analytical Biochemistry
• 出版年：2013
• 出版时间：1 September, 2013
• 年：2013
• 卷：440
• 期：1
• 页码：9-11
• 全文大小：628 K

文摘

We previously developed a molecular beacon-type probe to detect the strand scission in cellular base excision repair and found that the phosphodiester linkages in the fluorophore/quencher linkers were cleaved. This reaction was applied to a transfection reporter, which contained the unmodified phosphodiester in the linker to another type of fluorophore. After cotransfection of cells with the probe and the reporter, the signals were used to detect the incision and to confirm the proper transfection, respectively. This method will contribute to the prevention of false-negative results in experiments using molecular beacon-type probes.