Human Astrocytoma Cells Express Two Thromboxane A₂ Receptor Subtypes that Communicate with G_q and G₁₂

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• 作者：Shigeyoshi Honma ; Norimichi Nakahata ; Yasushi Ohizumi
• 关键词：Thromboxane A₂ ; thromboxane A₂ receptor subtype ; G_q ; 11 ; G₁₂ ; human astrocytoma cells
• 刊名：Prostaglandins and Other Lipid Mediators
• 出版年：1998
• 出版时间：February 1998
• 年：1998
• 卷：55
• 期：2-3
• 页码：159-168
• 全文大小：300 K

文摘

Thromboxane A₂ (TXA₂) receptor subtypes were examined with respect to mRNA and protein levels in 1321N1 human astrocytoma cells. Specific primers were synthesized for human placental and endothelial thromboxane A₂ (TXA₂) receptors. Reverse transcribed-polymerase chain reaction (PCR) (RT-PCR) using the specific primers amplified both human placental and endothelial TXA₂ receptor mRNAs in the cells. Anti-TXA₂ receptor antibody was raised in rabbits against a peptide sequence common to both TXA₂ receptors. The TXA₂ receptor proteins in the cells were partially purified by immunoaffinity chromatography with the anti-TXA₂ receptor antibody. The antibody recognized two proteins of 58 kDa and 55 kDa in the immunoaffinity-purified fraction. This fraction also contained Gα_q/11 and Gα₁₂. These results suggest that human astrocytoma cells express placental and endothelial TXA₂ receptor subtypes which may couple with G_q/11 and G₁₂.