Characterization of Argonaute2 gene from black tiger shrimp (Penaeus monodon) and its responses to immune challenges

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• 作者：Lishi Yang ; Xiaolan Li ; Song Jiang ; Lihua Qiu ; Falin Zhou ; Wenjing Liu ; Shigui Jiang
• 关键词：Argonaute2 ; Penaeus monodon ; RNAi pathway ; Bacteria ; Virus
• 刊名：Fish & Shellfish Immunology
• 出版年：January, 2014
• 年：2014
• 卷：36
• 期：1
• 页码：261-269
• 全文大小：2724 K

文摘

Argonaute2 binds to a short guide RNA (microRNA or short interfering RNA) and guides RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. Here we identified and characterized Argonaute2 from black tiger shrimp Penaeus monodon (designated as PmAgo2). The full-length cDNA of PmAgo2 contained a 5鈥?untranslated region (UTR) of 106聽bp, an open reading frame (ORF) of 2616聽bp and a 3鈥?UTR of 123聽bp. The predicted PmAgo2 protein is 99.4聽KDa with the theoretical isoelectric point of 9.54. PmAgo2 shared the highest similarity of amino acid with Marsupenaeus japonicus Argonaute2 and Litopenaeus vannamei Argonaute2, at 69.0% and 68.5%, respectively. Phylogenic analysis showed PmAgo2 clustered with shrimp Argonaute2, and closed to the group of insects. Real-time quantitative PCR showed that PmAgo2 was widely expressed in almost all examined tissues except eyestalk, with high expression in lymph and haemocyte. mRNA expression also revealed that PmAgo2 was significantly up-regulated by Staphylococcus aureus and White Spot Syndrome Virus (WSSV) in hepatopancreas. Furthermore, our study also confirmed that dsRNA and ssRNA homologous poly (I:C) and R848 activated the expression of PmAgo2. The result indicated that PmAgo2 responded to both bacterial infection and viral infection, especially, it may induce an ssRNA-mediated RNAi with other core members of siRNA pathway in black tiger shrimp.