Intranasal vaccination with a double mutant of staphylococcal enterotoxin C provides protection against Staphylococcus aureus infection

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• 作者：Dong-Liang Hu ; Katsuhiko Omoe ; Kouji Narita ; Jing-Chun Cui ; Kunihiro Shinagawa ; Akio Nakane
• 关键词：Enterotoxin ; Staphylococcus aureus ; Superantigen ; Intranasal vaccination ; Infection ; Vaccine ; Immune response
• 刊名：Microbes and Infection
• 出版年：2006
• 出版时间：November-December 2006
• 年：2006
• 卷：8
• 期：14-15
• 页码：2841-2848
• 全文大小：307 K

文摘

Staphylococcus aureus expresses a repertoire of factors including staphylococcal exotoxins (SEs), exoenzymes, and numerous cell-associated components that contribute to the pathogenesis of disease. We constructed and expressed a nontoxic double mutant SEC (dmSEC), devoid of superantigenic activity, and investigated the ability of intranasal vaccination with dmSEC plus cholera toxin (CT) adjuvant to protect mice against S. aureus infection. Mice were vaccinated with dmSEC and inoculated with a viable S. aureus clinical isolate strain. The survival rate in the immunized mice was higher, and bacterial counts in the organs were significantly lower than those in the control group. Intranasal vaccination with dmSEC induced the production of SEC-specific antibodies such as IgG1, IgG2b and IgA. dmSEC-vaccinated mice elicited significantly higher titers of interleukin-4 (IL-4) and IL-10, and lower levels of interferon-gamma (IFN-γ) after challenge with S. aureus compared with the control group. Furthermore, the sera from dmSEC-immunized mice significantly inhibited IFN-γ and tumor necrosis factor-α production in vitro. These results indicate that intranasal vaccination with dmSEC devoid of superantigenic properties induces systemic immune responses and provides protection against S. aureus infection.