RNA interference targeting against S100A4 suppresses cell growth and motility and induces apoptosis in human pancreatic cancer cells

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• 作者：Takahiro Tabata ; Nobukazu Tsukamoto ; Abbas Ali Imani Fooladi ; Sumitaka Yamanaka ; Toru Furukawa ; Masaharu Ishida ; Daisuke Sato ; Zhaodi Gu ; Hiroki Nagase ; Shinichi Egawa ; Makoto Sunamura ; Akira Horii
• 关键词：Invasion ; Pancreatic cancer ; PRDM2 ; RNAi ; S100A4 ; VASH1
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2009
• 出版时间：18 December 2009
• 年：2009
• 卷：390
• 期：3
• 页码：475-480
• 全文大小：511 K

文摘

S100A4 protein belongs to the S100 subfamily, which has grown to be one of the large subfamilies of the EF-hand Ca²⁺-binding proteins, and overexpression of S100A4 is suggested to associate with cell proliferation, invasion, and metastasis. We observed frequent overexpression of S100A4 in pancreatic cancer cell lines and further analyzed RNAi-mediated knockdown to address the possibility of its use as a therapeutic target for pancreatic cancer. The specific knockdown of S100A4 strongly suppressed cell growth, induced G2 arrest and eventual apoptosis, and decreased cell migration. Furthermore, microarray analyses revealed that knockdown of S100A4 induced expression of the tumor suppressor genes PRDM2 and VASH1. Our present results suggest the possibility that the inhibition of S100A4 can be utilized in antitumor applications for patients with pancreatic cancer.