- 作者：Go Koizumi ; Toshio Kumai ; Shunya Egawa ; Kentaro Yatomi ; Takeshi Hayashi ; Go Oda ; Keiichiro Ohba ; Shinichi Iwai ; Minoru Watanabe ; Naoki Matsumoto ; Katsuji Oguchi
- 关键词：Microarray analysis ; Atherosclerosis ; Esm1 ; Mkks ; Grem2 ; Retnlg
- 刊名：Life Sciences
- 出版年：2013
- 出版时间：10 October, 2013
- 年：2013
- 卷：93
- 期：15
- 页码：495-502
- 全文大小：1098 K
Aims
In recent years, there has been an increase in patients with arteriosclerosis and the risk of lifestyle-related diseases. However, the pathogenesis and medication of atherosclerosis have not been elucidated. We developed a rat model of lifestyle-related diseases by feeding a high-fat diet and 30 % sucrose solution (HFDS) to spontaneously hypertensive hyperlipidemic rats (SHHR) and reported that this model is a useful model of early atherosclerosis. In order to elucidate the pathogenesis of early atherosclerosis, we searched for atherosclerosis-related genes by microarray analysis using the aortic arch rat model of lifestyle-related diseases.Main methods
Four-month-old male Sprague-Dawley rats and SHHR were each divided into two normal diet (ND) groups and two HFDS groups. After a four-month treatment, the expression of mRNA in the aortic arch was detected using the oligo DNA microarray one-color method and quantified using real-time PCR.
Key findings
In this study, we detected 39 genes in microarray analysis. Esm1, Retnlb Mkks, and Grem2 showed particularly marked changes in gene expression in the SHHR-HFDS group. Compared with the SD-ND group, the SHHR-HFDS group had an increase in Mkks gene expression of about 26-fold and an approximately 22-fold increase in the expression of Grem2. Similarly, the expression of Esm1 increased by about 12-fold and that of Retnlg by about 10-fold as shown by quantitative real-time PCR.
Significance
This study suggested that these four genes might be important in early atherosclerosis development.