Novel screening system for high-affinity ligand of heredity vitamin D-resistant rickets-associated vitamin D receptor mutant R274L using bioluminescent sensor

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• 作者：Hiroki Mano^a ; Miyu Nishikawa^a ; ^b ; Kaori Yasuda^a ; Shinichi Ikushiro^a ; Nozomi Saito^c ; ¹ ; Daisuke Sawada^c ; ² ; Shinobu Honzawa^c ; ³ ; Masashi Takano^c ; Atsushi Kittaka^c ; Toshiyuki Sakaki^a ; ^b ; ^{tsakaki@pu-toyama.ac.jp}
• 关键词：VDR ; vitamin D receptor ; RXRα ; retinoid X receptor α ; LBD ; ligand binding domain ; VDRE ; vitamin D responsive element ; LucN ; N-terminal domain of luciferase ; LucC ; C-terminal domain of luciferase
• 刊名：The Journal of Steroid Biochemistry and Molecular Biology
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：167
• 期：Complete
• 页码：61-66
• 全文大小：615 K
• 卷排序：167

文摘

A fusion protein between split-luciferase and vitamin D receptor was constructed. Using this system, high-affinity ligands for the mutant VDR (R274L) were found. They might be potential medicines for the rickets caused by VDR (R274L).