Two new variants of the Rhodococcus equi virulence plasmid, 90 kb type III and type IV, recovered from a foal in Japan

详细信息    查看全文

• 作者：Takai ; Shinji ; Murata ; Norihisa ; Kudo ; Ryudai ; Narematsu ; Nobuhiro ; Kakuda ; Tsutomu ; Sasaki ; Yukako ; et. al.
• 关键词：Horse ; Plasmid ; Rhodococcus equi ; Variant ; Virulence
• 刊名：Veterinary Microbiology
• 出版年：2001
• 出版时间：October 1, 2001
• 年：2001
• 卷：82
• 期：4
• 页码：373-381
• 全文大小：179 K

文摘

This report describes the discovery of two new virulence plasmid types from a crossbred foal with Rhodococcus equi pneumonia in Kumamoto died with severe R. equi pneumonia and ulcerative enteritis. R. equi was isolated in large numbers and isolates from the foal were investigated for the presence of virulence-associated 15–17kDa antigens (VapA) by colony blotting, using the monoclonal antibody 10G5, and by gene coding for VapA by PCR. Plasmid DNAs extracted from the isolates were digested with restriction endonucleases BamHI, EcoRI, EcoT22I, and HindIII. The digestion patterns that resulted divided the plasmids of these isolates into two closely related types. The digestion patterns were then compared with eight representative virulence plasmid types (85kb types I, II, III and IV, 87kb types I and II, 90kb types I and II), which have already been reported. None of the EcoRI and EcoT22I digestion patterns of the eight representative plasmids matched those of the two plasmid types. We tentatively designated these new plasmid types as 90kb type III and type IV, since HindIII and BamHI digestion patterns of the two plasmid types were identical with those of a 90kb type I plasmid. This study, demonstrated that there are at least 10 distinct but closely related plasmids present in isolates from horses in the world.