- 作者：Pa-Fan Hsiao ; Cheng-Hsiang Hsiao ; Yang-Chih Lin ; Mei-Ping Tseng ; Tsen-Fang Tsai ; Shiou-Hwa Jee
- 关键词：laser capture microdissection ; mycosis fungoides ; polymerase chain reaction ; T-cell receptor γ ; Gene rearrangement
- 刊名：Journal of the Formosan Medical Association
- 出版年：2007
- 出版时间：2007
- 年：2007
- 卷：106
- 期：4
- 页码：265-272
- 全文大小：288 K
Background/purpose
Early mycosis fungoides (MF) is difficult to distinguish from other benign inflammatory dermatoses. We evaluated clonal T-cell receptor (TCR) γ Gene rearrangement by polymerase chain reaction (PCR) as a surrogate to histologic diagnosis in early MF.Methods
Twenty paraffin-embedded skin biopsies from nine patients diagnosed with MF were included. Two multiplex PCR encompassing various Vγ and Jγ regions were used to detect TCRγ Gene rearrangements. Histologic diagnoses were categorized as “diagnostic”, “consistent”, “suggestive”, or “nondiagnostic”. We compared TCRγ PCR results with histologic parameters to determine the differences between PCR-positive and PCR-negative groups.
Results
TCRg PCR was positive in 53 % (8/15) of the patch stage, in 100 % (2/2) of the plaque stage, and in 100 % (3/3) of the tumor stage. TCRγ PCR was positive in 50 % (4/8) of the specimens in both the diagnostic and consistent of MF groups, 71 % (5/7) in the suggestive of MF group. We found that inflammation was more severe in PCR-negative specimens. Papillary dermal fibrosis was common, and differed significantly between PCR-positive and PCR-negative groups (p = 0.01). T-cell monoclonality was detected in one nondiagnostic lesion in a patient with psoriasis and MF.
Conclusion
TCRγ PCR allows the diagnosis of MF in patients with lymphocyte-poor lesions, suggestive of MF pathologically. TCRg PCR is more likely to be negative with moderate to severe inflammation, particularly with papillary dermal fibrosis. We suggest that the ratio of malignant clonal to reactive T-cells is critical for MF diagnosis. [J Formos Med Assoc 2007;106(4):265-272]