The role of Alix in the proliferation of human glioma cells

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• 作者：Chengjin Zhao ; PhD^a ; ¹ ; Na Ban ; MS^b ; ¹ ; Shirong Dai ; PhD^a ; Xiubing Zhang ; PhD^a ; Li Zhang ; PhD^b ; Peng Xu ; MS^b ; Wenjuan Chen ; MS^c ; Jie Sun ; MS^c ; Zhen Bao ; MS^d ; Hao Chang ; MS^e ; Donglin Wang ; PhD^b ; Jianbing Ren ; PhD^a ; ^{jianbingren288@sina.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Glioma ; Alix ; Prognosis ; Proliferation ; Survival
• 刊名：Human Pathology
• 出版年：2016
• 出版时间：June 2016
• 年：2016
• 卷：52
• 期：Complete
• 页码：110-118
• 全文大小：1290 K

文摘

Apoptosis-linked-gene-2-interacting protein 1 (Alix) is involved in the endosome-lysosome system in the cytoplasm. The normal function of Alix may be altered by ALG-2 toward a destructive role during active cell death. Alix also may play a role in regulation of cell proliferation. However, the role of Alix in human glioma has not been elucidated yet. This study intended to clarify the relationship between Alix and glioma pathologic grades and its role in the proliferation of glioma cells. Our findings showed that Alix protein concentrations were significantly elevated in high-grade glioma tissue compared with low-grade glioma (P < .0001). Immunohistochemical study revealed that Alix was overexpressed in 75 resected glioma tissues and may forecast poor survival. Alix expression was increased in resting serum-stimulated glioma cells. Additionally, we reduced Alix expression in U251MG cells and then found that cell viability was decreased significantly when p21 expression increased. Colony formation assay and flow cytometry analysis demonstrated that reduced Alix expression may lead to growth inhibition and cell cycle arrest. In summary, our findings suggest that Alix plays an important role in the proliferation of glioma cells and may be a novel therapeutic target.