Pyruvic acid production by a thiamine auxotroph of Yarrowia lipolytica

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• 作者：Igor G. Morgunov ; Svetlana V. Kamzolova ; Oksana A. Perevoznikova ; Nadezda V. Shishkanova ; Tatiana V. Finogenova
• 关键词：Pyruvic acid production ; Yarrowia lipolytica ; Thiamine requirements ; Mechanism of pyruvic acid production
• 刊名：Process Biochemistry
• 出版年：2004
• 出版时间：30 July 2004
• 年：2004
• 卷：39
• 期：11
• 页码：1469-1474
• 全文大小：97 K

文摘

The thiamine-auxotrophic yeast Yarrowia lipolytica 374/4 was selected as a promising producer of pyruvic acid (PA). The thiamine concentration in the medium was shown to have a crucial effect on PA production by this strain. Under limitation of yeast growth by thiamine, the cell requirement in thiamine for PA production depended on a source of carbon and energy used. Cultivation conditions were established ensuring intense PA production by glycerol-grown yeast Y. lipolytica 374/4 (PA concentration, 61.3 g l⁻¹; specific rate of PA production, 76.3 mg PA (g cells h)⁻¹; mass yield coefficient (Y_PA), 0.71; and energy yield coefficient (η_PA), 0.53). A relationship between PA production and activities of enzymes involved in a primary glycerol oxidation, citrate and glyoxylate cycles (GCs) is discussed.