Dynamics of Membrane Nanotubulation and DNA Self-Assembly

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• 作者：T. Roopa ; N. Kumar ; S. Bhattacharya ; G.V. Shivashankar
• 刊名：Biophysical Journal
• 出版年：2004
• 出版时间：August 2004
• 年：2004
• 卷：87
• 期：2
• 页码：974-979
• 全文大小：184 K

文摘

A localized point-like force applied perpendicular to a vesicular membrane layer, using an optical tweezer, leads to membrane nanotubulation beyond a threshold force. Below the threshold, the force-extension curve shows an elastic response with a fine structure (serrations). Above the threshold the tubulation process exhibits a new reversible flow phase for the multilamellar membrane, which responds viscoelastically. Furthermore, with an oscillatory force applied during tubulation, broad but well-resolved resonances occur in the flow phase, presumably matching the time scales associated with the vesicle-nanotubule coupled system. These nanotubules, anchored to the optical tweezer also provide, for the first time, a direct probe of the real-time dynamics of DNA self-assembly on membranes. Our studies are a step in the direction of analyzing the dynamics of membrane self-assembly and artificial nanofluidic membrane networks.